Assessment of Cytotoxicity and DNA Damage Exhibited by Siloranes and Oxiranes in Cultured Mammalian Cells
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
The potential reactivity and structural properties of oxiranes (epoxides) are advantageous when considering polymers for medical devices. However, epoxy compounds are widely known to have genotoxic properties. The objective of the study was to evaluate the cytotoxicity and primary DNA damage effects induced by oxiranes and siloranes, silicon containing oxiranes. The siloranes, Ph-Sil, Tet-Sil, and Sil-Mix and the oxiranes Cyracure UVR-6105 and 1,3-bis[2-(2-oxiranylmethyl) phenoxy]pentane (OMP-5) were dissolved in organic solvents and dilutions containing less than 0.5% solvent were used in biological assays. The concentration that reduced the viability of 50% (TC(50)) of L929 cells was measured using the MTT assay and guided the selection of subtoxic doses for evaluation of DNA damage. Ph-Sil was more cytotoxic than OMP-5, Cyracure UVR-6105 and Sil-Mix. However, the TC(50) value of Tet-Sil could not be determined due to its poor solubility. DNA damage was evaluated in the sister chromatid exchange (SCE) assay with CHO cells, and the alkaline comet assay with L929 cells. In contrast to the siloranes, the oxiranes exhibited significant increases (p>0.05) in SCE frequencies and DNA migration relative to their solvent controls. Our findings support previous reports that siloranes have low genotoxic potential and can be suitable components for development of biomaterials.
Cell culture, Comet assay, DNA damage, Oxiranes, Silicon, Siloranes, Sister chromatid exchanges
Kostoryz EL, Zhu Q, Zhao H, Glaros A, Eick J. Assessment of Cytotoxicity and DNA Damage Exhibited by Siloranes and Oxiranes in Cultured Mammalian Cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2007; 634(1-2). doi: 10.1016/j.mrgentox.2007.07.003.