Document Type

Article

Publication Title

Medical Science Monitor Basic Research

Abstract

BACKGROUND

Obtaining high-quality images of cellular structures via immunofluorescence staining is critical for cellular localization studies. Often, these studies cannot be performed in parallel with certain oncology, virology, pharmacokinetic, and drug absorption studies due to model system technicalities requiring the cells to be cultured on porous membranes rather than glass or plastic.

MATERIAL AND METHODS

Here, we report a method of immunofluorescent staining of cells cultured on permeable membranes.

RESULTS

As proof of principle, HeLa cells grown on Transwell® membrane supports were stained with fluorescently labeled antibodies using this modified immunofluorescence staining method and visualized by fluorescent microscopy.

CONCLUSIONS

This protocol is a convenient alternative to staining cells on glass coverslips, thereby expanding the scope and applications of this important research tool.

DOI

10.12659/msmbr.900656

Publication Date

9-12-2016

Keywords

Diffusion Chambers, Culture, Fluorescent Antibody Technique, Direct, Staining and Labeling

ISSN

2325-4416

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