Fluorochroming Nuclei of Gold Chloride-Stained Motor Endings
Document Type
Article
Publication Title
Stain Technology
Abstract
Four millimeter cubes of muscle were soaked in 1% citric acid, 10 min; stained 60 min in 1% Au Cl3,; then reduced 24 hr in 20% formic acid. Small pieces of muscle were teased on a slide and flooded with a single fluorescent dye or combination of fluorochromes. Single saturated solutions of berberine, acridine orange, coriphosphine, and entozon granulate gave adequate results. Berberine in a dilution of 1:5; acridine orange, berberine, coriphosphine and rhodamine B in a dilution of 1:10 were suitable. Combinations of acridine orange and berberine, berberine and coriphosphine, rhodamine B and berberine, entoion granulate and berberine, primulin and berberine and a combination of berberine, coriphosphine and rhodamine B in dilutions of 1:10 in equal parts were successful. Dilutions were volumetric measurements from saturated solutions. After the application of one or more fluorochromes, the preparations were washed with distilled water and observed with a fluorescence microscope. The plasmodesmata of the motor endings and the axon appeared dark purple or black when stained with gold chloride alone or in combination. The nuclei appeared as light bodies throughout the field and the color varied according to the dyes used. There was no color differentation between connective tissue, nerve or muscle nuclei. The method quickly and consistently permits identification of the cellular elements of the motor end plate, connective tissue and muscle fibers.
DOI
10.3109/10520296209114567
Publication Date
1962
ISSN
1473-7760
Recommended Citation
Cole WV, Mielcarek J. Fluorochroming Nuclei of Gold Chloride-Stained Motor Endings. Stain Technology. 1962; 37(1). doi: 10.3109/10520296209114567.
