Document Type
Article
Publication Title
Genomics
Abstract
The saponins found in Panax japonicus, a traditional medicinal herb in Asia, exhibit high degrees of structural and functional similarity. In this study, metabolite analysis revealed that oleanolic acid-type and dammarane-type saponins were distributed unevenly in three tissues (rhizome_Y, rhizome_O, and secRoot) of P. japonicus. Single-molecule real-time (SMRT) sequencing and next generation sequencing (NGS) data revealed distinct and tissue-specific transcriptomic patterns relating to the production of these two types of saponins. In the co-expression network and hierarchical clustering analyses, one 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and two 1-deoxy-D-xylulose-5-phosphate synthase (DXS) etc. transcripts were found to be key genes associated with the biosynthesis of oleanolic acid and dammarane-type saponins in P. japonicus, respectively. In addition, cytochrome p450 (CYP) and UDP-glucuronosyltransferase (UGT) family proteins that serve as regulators of saponin biosynthesis-related genes were also found to exhibit tissue-specific expression patterns. Together these results offer a comprehensive metabolomic and transcriptomic overview of P. japonicus.
DOI
10.1016/j.ygeno.2020.06.045
Publication Date
11-2020
Keywords
Biosynthesis, Full-length transcriptome, Metabolome, Panax japonicus, Rhizoma and root tissues, Saponin distribution
ISSN
1089-8646
Recommended Citation
Xu R, Zhang J, You J, Gao L, Li Y, Zhang S, Zhu W, Shu S, Xiong C, Xiong H, Chen P, Guo J, Liu Z. Full-Length Transcriptome Sequencing and Modular Organization Analysis of Oleanolic Acid- and Dammarane-Type Saponins Related Gene Expression Patterns in Panax Japonicus. Genomics. 2020; 112(6). doi: 10.1016/j.ygeno.2020.06.045.
