A Bioenergetics Systems Evaluation of Ketogenic Diet Liver Effects

Authors

Lewis J. Hutfles, Kansas City University
Heather M. Wilkins, University of Kansas Medical Center
Scott J. Koppel, University of Kansas Medical Center
Ian W. Weidling, University of Kansas Medical Center
J. Eva Selfridge, University of Kansas Medical Center
Eephie Tan, University of Kansas Medical Center
John P. Thyfault, University of Kansas Medical Center
Chad Slawson, University of Kansas Medical Center
Aron W. Fenton, University of Kansas Medical Center
Hao Zhu, University of Kansas Medical Center
Russell H. Swerdlow, University of Kansas Medical Center

Document Type

Article

Publication Title

Applied Physiology, Nutrition, and Metabolism

Abstract

Ketogenic diets induce hepatocyte fatty acid oxidation and ketone body production. To further evaluate how ketogenic diets affect hepatocyte bioenergetic infrastructure, we analyzed livers from C57Bl/6J male mice maintained for 1 month on a ketogenic or standard chow diet. Compared with the standard diet, the ketogenic diet increased cytosolic and mitochondrial protein acetylation and also altered protein succinylation patterns. SIRT3 protein decreased while SIRT5 protein increased, and gluconeogenesis, oxidative phosphorylation, and mitochondrial biogenesis pathway proteins were variably and likely strategically altered. The pattern of changes observed can be used to inform a broader systems overview of how ketogenic diets affect liver bioenergetics.

DOI

10.1139/apnm-2017-0068

Publication Date

9-2017

Keywords

bioenergetics, bioénergétique, foie, hepatocyte, hépatocyte, ketogenic diet, liver, mitochondria, mitochondrie, régime cétogène

ISSN

1715-5320

Recommended Citation

Hutfles LJ, Wilkins HM, Koppel SJ, Weidling IW, Selfridge J, Tan E, Thyfault JP, Slawson C, Fenton AW, Zhu H, Swerdlow RH. A Bioenergetics Systems Evaluation of Ketogenic Diet Liver Effects. Applied Physiology, Nutrition, and Metabolism. 2017; 42(9). doi: 10.1139/apnm-2017-0068.